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Evaluation of Eosinophil Percentage and Charcot-Leyden Crystal Presence in Sputum Samples of Patients with and without Airway Hyperreactivity
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Research Article
VOLUME: 76 ISSUE: 4
P: 291-296
December 2023

Evaluation of Eosinophil Percentage and Charcot-Leyden Crystal Presence in Sputum Samples of Patients with and without Airway Hyperreactivity

J Ankara Univ Fac Med 2023;76(4):291-296
DOI: 10.4274/atfm.galenos.2023.07269
Duygu Öcal 1
Elif Oğuzman 1
Kutluay Acar 2
Helin Akyazılı 2
Aysu Arısoy 2
Begümhan Nur Ateş 2
Melisa Ethemoğlu 2
İrem Kar 3
Ebru Evren 1
1. Ankara Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye
2. Ankara Üniversitesi Tıp Fakültesi, Ankara, Türkiye
3. Ankara Üniversitesi Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Ankara, Türkiye
No information available.
No information available
Received Date: 25.04.2023
Accepted Date: 02.10.2023
Publish Date: 31.10.2023
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ABSTRACT

Objectives:

The aim of the study was to evaluate sputum obtained from asthmatic and non-asthmatic patients in terms of eosinophil percentage, presence of Charcot-Leyden Crystals (CLC) and culture results of sputum samples.

Materials and Methods:

In the study, 74 sputum culture samples sent to Ankara University Medical Faculty Hospital Cebeci Central Microbiology Laboratory between February 2021 and June 2021 were evaluated. Microscopic evaluations of the sputum samples were made with Gram and Giemsa stained preparations. The culture results of the sputum samples, which were accepted as “appropriate samples” according to the Gram stain results, were evaluated. Microorganisms were identified by conventional methods and automated systems VITEK II (bioMérieux, France) and MALDI-TOF MS (bioMérieux, France) were used as automated systems. Antimicrobial susceptibility tests were applied and evaluated in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing.

Results:

A total of 74 sputum samples were evaluated. Eosinophilia was detected in 40.9% of the sputum of asthmatic patients. While the mean percentage of eosinophils was found to be 0.63% in patients without a diagnosis of asthma (n=52), this rate was found as 3.04% in patients with asthma (n=22). CLC was detected in four of the sputum samples of those diagnosed with asthma. Microorganisms isolated from 16 (30.7%) of non-asthmatic patients in 6 (27.2%) of the sputum samples of patients with asthma were considered as causative agents. Pseudomonas aeruginosa is the most frequently isolated agent from the sputum samples. None of the panresistant microorganisms were not isolated.

Conclusion:

It was thought that it would be appropriate to evaluate both the percentage of eosinophils and the presence of CLC together as laboratory parameters that will help clinical diagnosis in cases with airway hyperreactivity such as asthma.

Keywords: Asthma, Sputum, Eosinophil, Charcot-Leyden Crystals

References

1
Kansal P, Nandan D, Agarwal S, et al. Correlation of induced sputum eosinophil levels with clinical parameters in mild and moderate persistent asthma in children aged 7-18 years. J Asthma. 2018;55:385-390.
2
Yancey SW, Keene ON, Albers FC, et al. Biomarkers for severe eosinophilic asthma. J Allergy Clin Immunol. 2017;140:1509-1518.
3
Bousquet J, Chanez P, Lacoste JY, et al. Eosinophilic inflammation in asthma. N Engl J Med. 1990;323:1033-1039.
4
Green RH, Brightling CE, McKenna S, et al. Asthma exacerbations and sputum eosinophil counts: a randomised controlled trial. Lancet. 2002;360:1715-1721.
5
Türktaş H. Asthma. In: Türktaş İ, Türktaş H, editörler. Astma. Ankara: Bozkır matbaacılık.; 1998. s. 1-59.
6
Su J. A Brief History of Charcot-Leyden Crystal Protein/Galectin-10 Research. Molecules. 2018;23:2931.
7
Persson EK, Verstraete K, Heyndrickx I, et al. Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science. 2019;364:eaaw4295.
8
Ueki S, Tokunaga T, Melo RCN, et al. Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death. Blood. 2018;132:2183-2187.
9
Eraksoy H. Pnömoniler. In: Wilke Topçu A, Söyletir G, Doğanay M (eds.) Enfeksiyon Hastalıkları ve Mikrobiyolojisi, Sistemlere Göre Enfeksiyonlar 3. Baskı, İstanbul: Nobel Tıp Kitabevleri.; 2008. s. 788-820.
10
Kurutepe S, Ecemiş T, Ozgen A,. Toplum Kökenli Pnömonisi Olan Erişkin Hastalarda Konvansiyonel ve Multipleks PCR Yöntemleriyle Bakteriyel Etiyolojinin Araştırılması. Mikrobiyol Bul. 2012;46:523-531.
11
Hörü G, Ecemiş T, Kurutepe S, et al. Hastanede Yatan Hastaların Alt Solunum Yolu Örneklerinden İzole Edilen Gram Negatif Bakterilerde Antimikrobiyal Direnç. Klimik Dergisi. 2011; 24:112-115.
12
The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0. 2021. http://www.eucast.org.
13
Westerhof GA, Korevaar DA, Amelink M, et al. Biomarkers to identify sputum eosinophilia in different adult asthma phenotypes. Eur Respir J. 2015;46:688-696.
14
Bandyopadhyay A, Roy PP, Saha K, et al. Usefulness of induced sputum eosinophil count to assess severity and treatment outcome in asthma patients. Lung India. 2013;30:117-123.
15
Rodríguez-Alcázar JF, Ataide MA, Engels G, et al. Charcot-Leyden Crystals Activate the NLRP3 Inflammasome and Cause IL-1β Inflammation in Human Macrophages. J Immunol. 2019;202:550-558.
16
Martin RJ, Kraft M, Chu HW, et al. A link between chronic asthma and chronic infection. J Allergy Clin Immunol. 2001;107:595-601.
17
Castillo JR, Peters SP, Busse WW. Asthma Exacerbations: Pathogenesis, Prevention, and Treatment. J Allergy Clin Immunol Pract 2017;5:918-927.
18
Altay Koçak A, Yayla B, Güçlü AÜ, et al. Adana’da bir üniversite hastanesinde izole edilen solunum yolu patojenleri ve antibiyotik direnç profillerinin değerlendirilmesi. Türk Mikrobiyoloji Cem Derg. 2019;49:226-232.
19
Kılıç T, Yıldırım Z, Özerol İ, et al. Kronik obstruktif akciğer hastalığında bakteriyel kolonizasyon ve akut alevlenme sıklığının ilişkisi Yeni Tıp Dergisi. 2014;31:180-185
20
UAMDSS. Ulusal Antimikrobiyal Direnç Sürveyans Sistemi, 2016 Yıllık Raporu, Türkiye Halk Sağlığı Kurumu, Sağlık Bakanlığı Ankara. http://uamdss.thsk.gov.tr
21
Flamm RK, Sader HS, Farrell DJ, et al. Antimicrobial activity of ceftaroline tested against drug-resistant subsets of Streptococcus pneumoniae from U.S. medical centers. Antimicrob Agents Chemother. 2014;58:2468-2471.
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