Comparing The Different Fixatives For Examination of Liver Tissue Ultrastructure
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Research Article
P: 75-79
August 2011

Comparing The Different Fixatives For Examination of Liver Tissue Ultrastructure

J Ankara Univ Fac Med 2011;64(2):75-79
1. Ufuk Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji Anabilim Dalı
2. Ankara Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji Anabilim Dalı
No information available.
No information available
Received Date: 14.03.2011
Accepted Date: 20.07.2011
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ABSTRACT

Aim:

It is important to examine liver tissue ultrastructure in pathologies of infections and storage diseases. The aim of this study was to assess the effects and advantages of various fixatives on liver ultrastructure and to minimize the methodological errors during the fixation of liver tissue for transmission electron microscopy.

Materials and Methods:

Five Wistar rats were used. After the rats were perfused with 4% paraformaldehyde (PFA), tissues were extracted and immersed with four different fixation techniques:

I- Trump’s solution 4% paraformaldehyde (PFA) and 1% glutaraldehyde (G)

II- 2% F and 2.5% G with 2.5 mM CaCl

III- 2% PFA and 2.5% G

IV- 2.5% G in 0.1M Cacodylate buffer

Samples were embedded, cut, stained and examined with transmission electron microscope.

Samples were scored and analyzed.

Results:

The plasma membrane and cellular junctions were better preserved in group I and II. The best mitochondria structure was in group IV. The total score rank from the highest to the lowest was as follows: Group I, IV, II, and III.

Conclusion:

The results for the samples fixed in Trump’s fixative were better than those achieved by other fixing agents.

Keywords:
Fixatives
liver
technique
transmission electron microscope (T.E.M.)